ID   PFFRM      standard; DNA; INV; 843 BP.
XX
AC   L40835;
XX
NI   g725288
XX
DT   13-APR-1995 (Rel. 43, Created)
DT   24-AUG-1995 (Rel. 44, Last updated, Version 2)
XX
DE   Plasmodium falciparum (clones BRA3-5b,-19a,-14b,-28e) liver
DE   stage-specific antigen 1 (LSA-1) gene, 3' end of cds.
XX
KW   exoerythrocytic; liver stage-specific antigen;
KW   liver stage-specific antigen 1; Malaria.
XX
OS   Plasmodium falciparum (malaria parasite)
OC   Eukaryotae; mitochondrial eukaryotes; Alveolata; Apicomplexa;
OC   Haemosporida; Plasmodium.
XX
RN   [1]
RP   1-843
RX   MEDLINE; 96065765.
RA   Yang C., Shi Y.P., Udhayakumar V., Alpers M.P., Povoa M.M.,
RA   Hawley W.A., Collins W.E., Lal A.A.;
RT   "Sequence variations in the non-repetitive regions of the liver
RT   stage-specific antigen-1 (LSA-1) of Plasmodium falciparum from
RT   field isolates";
RL   Mol. Biochem. Parasitol. 71:291-294(1995).
XX
DR   SPTREMBL; Q25853; Q25853.
XX
CC   1. Last author is Altaf A. Lal 2. The following clones have the sam
CC   e nucleotide sequence: BRA3-5b; BRA3-19a; BRA3-14b (one silent
CC   change at 5181, A to G) and BRA3-28e (one silent change at 5040, T
CC   to C) Clone name abbreviations: KEN for Kenya; PNG for Papua New
CC   Guinea and BRA for Brazil. For each clone, the number following the
CC   KEN, PNG or BRA represents group number, the number following the
CC   '-' denotes the patient's blood sample number and the lower case
CC   letter represents recombinant clones from the corresponding
CC   isolates. NCBI gi: 725288
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..843
FT                   /organism="Plasmodium falciparum"
FT                   /clone="BRA3-14b"
FT                   /isolate="Kenyan"
FT                   /dev_stage="blood stage parasite"
FT                   /sequenced_mol="DNA"
FT   source          1..843
FT                   /organism="Plasmodium falciparum"
FT                   /clone="BRA3-19a"
FT                   /isolate="Kenyan"
FT                   /dev_stage="blood stage parasite"
FT                   /sequenced_mol="DNA"
FT   CDS             <1..843
FT                   /gene="LSA-1"
FT                   /note="NCBI gi: 950674"
FT                   /codon_start=1
FT                   /product="liver stage-specific antigen 1"
FT                   /db_xref="PID:g950674"
FT                   /db_xref="SPTREMBL:Q25853"
FT                   /translation="RKADTKKNLERKKEHGDVLAEDLYGRLEIPVIELPSEN
ERGYYIP
FT                   HQSSLPQDNRGNSRDSKEISIIEKTNRESITTNVEGRRDIHKGHLEEKKDGS
IKPEQKE
FT                   DKSADIQNHTLETVNISDVNDFQISKYEDEISAEYDDSLIDEEEDDEDLDEF
KPIVQYD
FT                   NFQDEENIGIYKELEDLIEKNENLDDLDEGIEKSSEELSEEKIKKGKKYEKT
KDNNFKP
FT                   NDKSLYDEHIKKYKNVKQVNKEKEKFIKSLFHIFDGDNEILQIVDELSEDIT
KYFMKL"
FT   source          1..843
FT                   /organism="Plasmodium falciparum"
FT                   /clone="BRA3-5b"
FT                   /organism="Plasmodium falciparum"
FT                   /clone="BRA3-5b"
FT                   /isolate="Kenyan"
FT                   /dev_stage="blood stage parasite"
FT                   /sequenced_mol="DNA"
FT   source          1..843
FT                   /organism="Plasmodium falciparum"
FT                   /clone="BRA3-28e"
FT                   /isolate="Kenyan"
FT                   /dev_stage="blood stage parasite"
FT                   /sequenced_mol="DNA"
FT   source          1..843
FT                   /organism="Plasmodium falciparum"
FT                   /clone="BRA3-29b"
FT                   /isolate="Kenyan"
FT                   /dev_stage="blood stage parasite"
FT                   /sequenced_mol="DNA"
FT   mutation        replace(75,"c")
FT                   /gene="LSA-1"
FT                   /note="clone BRA3-28e"
FT   mutation        replace(216,"g")
FT                   /gene="LSA-1"
FT                   /note="clone BRA3-14b"
XX
SQ   Sequence 843 BP; 397 A; 78 C; 153 G; 215 T; 0 other;
     aggaaggctg atacgaaaaa aaatttagaa agaaaaaagg aacatggaga tgtattagca        60
     gaggatttat atggtcgttt agaaatacca gttatagaac ttccatcaga aaatgaacgt       120
     ggatattata taccacatca atcttcttta cctcaggaca acagagggaa tagtagagat       180
     tccaaggaaa tatctataat agaaaaaaca aatagagaat ctattacaac aaatgttgaa       240
     ggacgaaggg atatacataa aggacatctt gaagaaaaga aagatggttc aataaaacca       300
     gaacaaaaag aagataaatc tgctgacata caaaatcata cattagagac agtaaatatt       360
     tctgatgtta atgattttca aataagtaag tatgaggatg aaataagtgc tgaatatgac       420
     gattcattaa tagatgaaga agaagatgat gaagacttag acgaatttaa gcctattgtg       480
     caatatgaca atttccaaga tgaagaaaac ataggaattt ataaagaact agaagatttg       540
     atagagaaaa atgaaaattt agatgattta gatgaaggaa tagaaaaatc atcagaagaa       600
     ttatctgaag aaaaaataaa aaaaggaaag aaatatgaaa aaacaaagga taataatttt       660
     aaaccaaatg ataaaagttt gtatgatgag catattaaaa aatataaaaa tgttaagcag       720
     gttaataagg aaaaggaaaa attcataaaa tcattgtttc atatatttga cggagacaat       780
     gaaattttac agatcgtgga tgagttatct gaagatataa ctaaatattt tatgaaacta       840
     taa                                                                     843
//