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P.falciparum cloned lines:

Clone Origin Drug Sensitivity Protein markers Isoenzymes 2D-gel proteins

3D7A Netherlands, Clone from isolate NF54; Walliker et al., 1987, Science, 236, 1661-1666 CQ 10-7 Pyr 10-9 5.1 - 7.3- 9.2+ 9.5- 12.2+ 12.1- 10.4+ 9.4- 12.3- 9.21- GPI-1 ADA-1 PEP-1 A-2 C-2 D-2 E-3 F-2 G-1 K-3

3D7B Netherlands,

3D7A after mosquito transmission CQ 10-7 Pyr 10-9 5.1 - 7.3- 9.2+ 9.5- 12.2+ 12.1- 10.4+ 9.4- 12.3- 9.21- GPI-1 ADA-1 PEP-1 A-2 C-2 D-2 E-3 F-2 G-1 K-3

HB3A Honduras, Clone of H-1, produced by W. Trager. (Walliker et al., 1987, Science, 236-1661-1666). CQ 10-7 Pyr 10-7 5.1+ 9.2- 12.2- 10.4- 12.3- 7.3+ 9.5- 12.1- 9.4- 9.21+ GPI-1 ADA-2 PEP-1 A-2 D-4 F-2 K-1 C-6 E-4 G-2

HB3B Honduras = HB3A after mosquito transmission CQ 10-7 Pyr 10-7 5.1+ 9.2- 12.2- 10.4- 12.3- 7.3+ 9.5- 12.1- 9.4- 9.21+ GPI-1 ADA-2 PEP-1 A-2 D-4 F-2 K-1 C-6 E-4 G-2

7G8 Brazil. Clone derived from isolate IMTM22 (Burkot et al., 1984, Trans Roy Soc Trop Med Hyg 78, 339-341) CQ10-6 Pyr10-5 5.1- 9.2- 12.2- 10.4+ 12.3+ 7.3- 9.5+ 12.1- 9.4- 9.21+ GPI-1 ADA-1 PEP-1 Not tested

T9-94 Thailand. Clone from isolate T9. (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245) CQ5x10-7 Pyr10-9 5.1+ 9.2- 12.2- 10.4- 12.3+ 7.3+ 9.5- 12.1- 9.4- 9.21- GPI-1 ADA-1 PEP-1 A-4 D-5 F-3 K-6 C-2 E-2 G-2

T9-96 Thailand. Clone from isolate T9. (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245) CQ10-7 Pyr10-9 5.1+ 9.2+ 12.2+ 10.4+ 12.3+ 7.3- 9.5- 12.1+ 9.4- 9.21+ GPI-2 ADA-1 PEP-2 A-2 D-4 F-2 K-3 C-1 E-3 G-2

T9-98 Thailand. Clone from isolate T9 (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245) CQ10-7 5x10-5 5.1+ 9.2+ 12.2+ 10.4+ 12.3+ 7.3- 9.5- 12.1+ 9.4- 9.21+ GPI-2 ADA-1 PEP-2 A-2 D-4 F-2 K-3 C-1 E-3 G-2

T9-101 Thailand. Clone from isolate T9 (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245) CQ10-6 5x10-5 5.1+ 9.2+ 12.2+ 10.4+ 12.3+ 7.3- 9.5- 12.1- 9.4+ 9.21+ GPI-1 ADA-2 PEP-1 A-1 D-4 F-2 K-6 C-2 E-3 G-2

T9-102 Thailand. Clone from isolate T9. (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245) CQ10-6 Pyr5x10-5 5.1- 9.2+ 12.2- 10.4+ 12.3+ 7.3- 9.5- 12.1+ 9.4+ 9.21+ GPI-1 ADA-1 PEP-1 A-1 D-5 F-2 K-6 C-2 E-3 G-2

Clone	Origin	Drug Sensitivity	Protein markers	Isoenzymes	2D-gel proteins
3D7A	Netherlands, Clone from isolate NF54; Walliker et al., 1987, Science, 236, 1661-1666	CQ 10-7 Pyr 10-9	5.1 - 7.3- 9.2+ 9.5- 12.2+ 12.1- 10.4+ 9.4- 12.3- 9.21-	GPI-1 ADA-1 PEP-1	A-2 C-2 D-2 E-3 F-2 G-1 K-3
3D7B	Netherlands,
3D7A	after mosquito transmission	CQ 10-7 Pyr 10-9	5.1 - 7.3- 9.2+ 9.5- 12.2+ 12.1- 10.4+ 9.4- 12.3- 9.21-	GPI-1 ADA-1 PEP-1	A-2 C-2 D-2 E-3 F-2 G-1 K-3
HB3A	Honduras, Clone of H-1, produced by W. Trager. (Walliker et al., 1987, Science, 236-1661-1666).	CQ 10-7 Pyr 10-7	5.1+ 9.2- 12.2- 10.4- 12.3- 7.3+ 9.5- 12.1- 9.4- 9.21+	GPI-1 ADA-2 PEP-1	A-2 D-4 F-2 K-1 C-6 E-4 G-2
HB3B	Honduras = HB3A after mosquito transmission	CQ 10-7 Pyr 10-7	5.1+ 9.2- 12.2- 10.4- 12.3- 7.3+ 9.5- 12.1- 9.4- 9.21+	GPI-1 ADA-2 PEP-1	A-2 D-4 F-2 K-1 C-6 E-4 G-2
7G8	Brazil. Clone derived from isolate IMTM22 (Burkot et al., 1984, Trans Roy Soc Trop Med Hyg 78, 339-341)	CQ10-6 Pyr10-5	5.1- 9.2- 12.2- 10.4+ 12.3+ 7.3- 9.5+ 12.1- 9.4- 9.21+	GPI-1 ADA-1 PEP-1	Not tested
T9-94	Thailand. Clone from isolate T9. (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245)	CQ5x10-7 Pyr10-9	5.1+ 9.2- 12.2- 10.4- 12.3+ 7.3+ 9.5- 12.1- 9.4- 9.21-	GPI-1 ADA-1 PEP-1	A-4 D-5 F-3 K-6 C-2 E-2 G-2
T9-96	Thailand. Clone from isolate T9. (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245)	CQ10-7 Pyr10-9	5.1+ 9.2+ 12.2+ 10.4+ 12.3+ 7.3- 9.5- 12.1+ 9.4- 9.21+	GPI-2 ADA-1 PEP-2	A-2 D-4 F-2 K-3 C-1 E-3 G-2
T9-98	Thailand. Clone from isolate T9 (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245)	CQ10-7 5x10-5	5.1+ 9.2+ 12.2+ 10.4+ 12.3+ 7.3- 9.5- 12.1+ 9.4- 9.21+	GPI-2 ADA-1 PEP-2	A-2 D-4 F-2 K-3 C-1 E-3 G-2
T9-101	Thailand. Clone from isolate T9 (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245)	CQ10-6 5x10-5	5.1+ 9.2+ 12.2+ 10.4+ 12.3+ 7.3- 9.5- 12.1- 9.4+ 9.21+	GPI-1 ADA-2 PEP-1	A-1 D-4 F-2 K-6 C-2 E-3 G-2
T9-102	Thailand. Clone from isolate T9. (Thaithong et al., 1984. Trans Roy Soc Trop Med Hyg 78, 242-245)	CQ10-6 Pyr5x10-5	5.1- 9.2+ 12.2- 10.4+ 12.3+ 7.3- 9.5- 12.1+ 9.4+ 9.21+	GPI-1 ADA-1 PEP-1	A-1 D-5 F-2 K-6 C-2 E-3 G-2