Multiple sequence alignment of RAP-1 alleles in Plasmodium falciparum and homologues in other Plasmodium species 1 50 India D SLVI IFHVLFRNVA DGINVNGDNN YGKTIINNDF NFDDYNYWTP reichinowi MSFYLGSLVI IFHVLFRNVA DGINVNGDNN YGKTIIHNDF NFDDYNYWTP K1 MSFYLGSLVI IFHVLFRNVA DGINVNGDNN YGKTIINNDF NFDDYNYWTP Honduras I MSFYLGSLVI IFHVLFRNVA DGINVNGDNN YGKTIINNDF NFDDYNYWTP *consensus* MSFYLGSLVI IFHVLFRNVA DGINVNGDNN YGKTIInNDF NFDDYNYWTP 51 100 India D INKKEFLNSY ENEFSSESFL ENKSSVDDGN INLTDTSTSN KSSKKGHGRS reichinowi INKKEFLNSY EDEFSSESFL ENKSSVDDGN INLTDTSASN KSSRKGHGRS K1 INKKEFLNSY EDEFSSESFL ENKSSVDDGN INLTDTSTSN KSSKKGHGRS Honduras I INKKEFLNSY EDKFSSESFL ENKSSVDDGN INLTDTSTSN KSSKKGHGRS *consensus* INKKEFLNSY EdeFSSESFL ENKSSVDDGN INLTDTStSN KSSkKGHGRS 101 150 SierraLeone ASPSVVK TSTPSGTQTS GLKSSSPSST Tanzania ASPSVVK TSTPSGTQTS GSKSSSPSST India D RVRSASAAAI LEEDDSKDDM EFKASPSVVK TSTPSGTQTS GLKSSSPSIT reichinowi RVRSASAAAI LEEDDSKDDI EFKASPSVVK TSTPTGTHTS GSKSSSPSST K1 RVRSASAAAI LEEDDSKDDM EFKASPSVVK TSTPSGTQTS GLKSSSPSST Honduras I RVRSASAAAI LEEDDSKDDM EFKASPSVVK TSTPSGTQTS GSKSSSPSST *consensus* RVRSASAAAI LEEDDSKDDm EFKASPSVVK TSTPsGTqTS G.KSSSPSsT 151 200 SierraLeone KSSSPSNVKS ASPHGESNSS EESTTKSSKR SASXAGIVGA DEEAPPAPKN Tanzania KSSSPSNVKS ASPHGESNSS EESTTKSSKR SASVAGIVGA DEEAPPAPKN India D KSSSPSNVKS ASPHGESNSS EESTTKSSKR SASVAGIVGA DEEAPPAPKN reichinowi KSSSPSNVKT ASPHGESNSS EESTSKSSKR SASVSGIVGA DEEVPPAPKN K1 KSSSPSNVKS ASPHGESNSS EESTTKSSKR SASVAGIVGA DEEAPPAPKN Honduras I KSSSPSNVKS ASPHGESNSS EESTTKSSKR SASVAGIVGA DEEAPPAPKN *consensus* KSSSPSNVKs ASPHGESNSS EESTtKSSKR SASvaGIVGA DEEaPPAPKN 201 250 SierraLeone TLTPLEELYP TNVNLFNYKY SLNNMEENIN ILKNEGDLVA QKEEFEYDEN Tanzania TLTPLEELYP TNVNLFNYKY SLNNMEENIN ILKNEGDLVA QKEEFEYDEN India D TLTPLEELYP TNVNLFNYKY SLNNMEENIN ILKNEGDLVA QKEEFEYDEN reichinowi TLTPLEELYP TNVNLFNYKY SLNNMEENIN ILKNEGQLVA QKEEFDYDEN K1 TLTPLEELYP TNVNLFNYKY SLNNMEENIN ILKNEGDLVA QKEEFEYDEN Honduras I TLTPLEELYP TNVNLFNYKY SLNNMEENIN ILKNEGDLVA QKEEFEYDEN *consensus* TLTPLEELYP TNVNLFNYKY SLNNMEENIN ILKNEGdLVA QKEEFeYDEN 251 300 SierraLeone MEKAKQDKKK ALEKIGKESD EAPFMFSENK FLENQVKERN VAGS Tanzania MEKAKQDKKK ALEKIGKESD EEPFMFSENK FLENQVKERN VAGS India D MEKAKQDKKK ALEKIGKQSD EEPFMFSENK FLENQVKERN VAGSFSRF reichinowi MEKAKEEKQK ALEKIGKETD EEPFMFTDDK FLENQVKERN VAGSFSRFFS K1 MEKAKQDKKK ALEKIGKQSD EEPFMFSENK FLENQVKERN VAGSFSRFFS Honduras I MEKAKQDKKK ALEKIGKESD EEPFMFSENK FLENQVKERN VAGSFSRFFS *consensus* MEKAKqdKkK ALEKIGKesD EePFMFsenK FLENQVKERN VAGSFSRFFS 301 350 reichinowi KLNPFKKDEV IEKTEVSKKT YSGIGFNVND KEAKILGVGA TYQEYPETML K1 KLNPFKKDEV IEKTEVSKKT FSGIGFNLTD KEAKVLGVGA TYQEYPETML Honduras I KLNPFKKDEV IEKTEVSKKT FSGIGFNLTE KEAKVLGVGV TYQEYPETML *consensus* KLNPFKKDEV IEKTEVSKKT fSGIGFNltd KEAKvLGVGa TYQEYPETML 351 400 reichinowi YNCPNNSHLF DTISSLQGRL IDIKKRENMI STTFEQQKEC LKNMGVLDLE K1 YNCPNNSNLF DTIESLQGRI IDIKKRESMI STTFEQQKEC LKNMGVLDLE Honduras I YNCPNNSNLF DTIESLQGRI IDIKKRESMI STTFEQQKEC LKNMGVLDLE *consensus* YNCPNNSnLF DTIeSLQGRi IDIKKREsMI STTFEQQKEC LKNMGVLDLE 401 450 reichinowi LKDTECKFGT CIGSFGEHHL RLYEFENDLF KFHPNIDYLT LADGYKLQKN K1 LNDTQCKFGT CIGSFGEHHL RLYEFENDLF KFHPNIDYLT LADGYKLQKN Honduras I LNDTQCKFGT CIGSFGEHHL RLYEFENDLF KFHPNIDYLT LADGYKLQKN *consensus* LnDTqCKFGT CIGSFGEHHL RLYEFENDLF KFHPNIDYLT LADGYKLQKN 451 500 reichinowi HIYELSHVNF CLLNPKTLEE FLKKKEIKDL MGGDDLIKYK ENFDNFMSIS K1 HIYELSHVNF CLLNPKTLEE FLKKKEIKDL MGGDDLIKYK ENFDNFMSIS Honduras I HIYELSHVNF CLLNPKTLEE FLKKKEIKDL MGGDDLIKYK ENFDNFMSIS *consensus* HIYELSHVNF CLLNPKTLEE FLKKKEIKDL MGGDDLIKYK ENFDNFMSIS 501 550 reichinowi ITCHIESLIY DDIEASQDVA AVLKIAKSKL HVITSGLSYK ARKLVYKIYS K1 ITCHIESLIY DDIEASQDIA AVLKIAKSKL HVITSGLSYK ARKLVYKIYS Honduras I ITCHIESLIY DDIEASQDIA AVLKIAKSKL HVITSGLSYK ARKLVYKIYS consensus ITCHIESLIY DDIEASQDiA AVLKIAKSKL HVITSGLSYK ARKLVYKIYS 551 600 reichinowi EIQKNPDELY EKLTWIYDNI YMLKRYYTAY ALEGVCSYIE NDKTQMDTEL K1 EIQKNPDELY EKLTWIYDNI YMIKRYYTAY ALEGVCSYLE HDKSQMYTEL Honduras I EIQKNPDELY EKLTWIYDNI YMIKRYYTAY ALEGVCSYLE HDKSQMYTEL *consensus* EIQKNPDELY EKLTWIYDNI YMiKRYYTAY ALEGVCSYlE hDKsQMyTEL 601 650 India J2 SCFKNV IVYNAIISGI HEKIKHFLKL VPRHNFLLDY India J1 KNV IVYNAIISGI HEKIKHFLKL VPRHNFLLDY India D YSSCFKNV IVYNAIISGI HEKIKHFLKL VPRHNFLLDY India C YSSCFKNV IVYNAIISGI HEKIKHFLKL VPRHNFLLDY reichinowi HIYNKIVDSV RYYSSCFKNV IVYNAIMSGI HEKIKHFLKL VPRQNFLLDY K1 HIYNKIVDSV RYYSSCFKNV IVYNAIISGI HEKIKHFLKL VPRHNFLLDY Honduras I HIYNKIVDSV RYYSSCFKNV IVYNAIISGI HEKIKHFLKL VPRHNFLLDY *consensus* HIYNKIVDSV RYYSSCFKNV IVYNAIiSGI HEKIKHFLKL VPRhNFLLDY 651 700 India J2 HFNSIFEKEI KPAKKYSTSH IYFDPTVASY AYYNLDRRTM VTIINDYFEA India J1 HFNSVFEKEI KPAKKYSTSH IYFDPTVASY AYYNLDRRTM VTIINDYFEA India D HFNSIFEKEI KPAKKYSTSH IYFDPTVASY AYYNLDRRTM VTIINDYFEA indc-c HFNSIFEKEI KPAKKYSTSH IYFDPTVASY AYYNLDRRTM VTIINDYFEA reichinowi HFNSIFEKEI KPVKKYSTSH VYYDPTVASY AYYNLDRRTM VTIINDYFEA K1 HFNSIFEKEI KPAKKYSTSH IYFDPTVASY AYYNLDRRTM VTIINDYFEA Honduras I HFNSIFEKEI KPAKKYSTSH IYFDPTVASY AYYNLDRRTM VTIINDYFEA consensus HFNSiFEKEI KPaKKYSTSH iYfDPTVASY AYYNLDRRTM VTIINDYFEA 701 750 India J2 KKKELTVIVS RMKTDMLSIQ NEESKIPNDK SANSKLATRL MKKFKAEIRD India J1 KKKELTVIVS RMKTDMLSLQ NEESKIPNDK SANSKLATRL MKKFKAEIRD India D KKKELTVIVS RMKTDMLSLQ NEESKIPNDK SANSKLATRL MKKFKAEIRD India C KKKELTVIVS RMKTDMLSLQ NEESKIPNDK SANSKLATRL MKKFKAEIRD reichinowi KKKELTVIVS RMKTDMLSLQ NDESKIPNDK SANSKLATRL MRKFKAEIRD K1 KKKELTVIVS RMKTDMLSLQ NEESKIPNDK SANSKLATRL MKKFKAEIRD Honduras I KKKELTVIVS RMKTDMLSLQ NEESKIPNDK SANSKLATRL MKKFKAEIRD *consensus* KKKELTVIVS RMKTDMLSlQ NeESKIPNDK SANSKLATRL MkKFKAEIRD 751 782 India J2 FFKEMRIQYA KLINIRYRSH LKKNY India J1 FFKEMRIQYA KLINIRYRSH India D FFKEMRIQYA KLINIRYRSH LKKNY India C FFKEMRIQYA KLINIRYRSH LKKNY reichinowi FFKEMRIQYA KLINIRYKSH LKKNYFAFKR LD K1 FFKEMRIQYA KLINIRYRSH LKKNYFAFKR LD Honduras I FFKEMRIQYA KLINIRYRSH LKKNYFAFKR LD *consensus* FFKEMRIQYA KLINIRYrSH LKKNYFAFKR LD Notes: 1. Both Ala and Val were observed at X (position 184) in Sierra Leone. References: 1. Ridley, R. G., Takacs, B., Lahm, H.-W., Delves, C. J., Goman, M., Certa, U., Matile, H., Woollett, G. R., and Scaife, J. G. (1990) Mol. Biochem. Parasitol. 41, 125-134 2. Howard, R. F. (1992) Mol. Biochem. Parasitol. 51, 327-330 3. Howard, R. F., Jensen, J. B., and Franklin, H. L. (1993) Infect. Immun. 61, 2960-2965 4. Howard, R. F. and Peterson, C. (1996) Mol. Biochem. Parasitol. 77, 95-98 5. Plasmodium reichinowii coding sequence: accession no. U20986